Medchemexpress LLC PDGF-AA Human His 10ug
Recombinant human PDGF-AA (His) is a lyophilized preparation of the mature PDGF-A isoform (residues S87-T211) produced in Escherichia coli for use in cell-based and biochemical assays. It is supplied as a highly pure, active growth factor suitable for studies of PDGF receptor signaling, cell proliferation, migration, and wound-healing.
- Contains the mature PDGF-A sequence (S87-T211) with an N-terminal 6xHis tag.
- Expressed in Escherichia coli and provided as lyophilized powder.
- Apparent molecular weight ~16-18 kDa; predicted mass 15.9 kDa.
- Purity greater than 95% by reducing SDS-PAGE.
- Low endotoxin level (<1 EU/μg) suitable for cell assays.
- Formulated and lyophilized from filtered buffer (HCl or Tris-HCl with NaCl and arginine).
- Stable stored at -20°C; after reconstitution stable at 4°C for 1 week or -20°C for longer with carrier protein.
- Biological activity ED50 ≈53.4 ng/mL in NIH-3T3 proliferation assay.